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hamster anti mouse cd31 mab (Bio-Rad)

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Bio-Rad hamster anti mouse cd31 mab
Hamster Anti Mouse Cd31 Mab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 21 article reviews

hamster anti mouse cd31 mab - by Bioz Stars, 2026-03

93/100 stars

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Journal: Communications Biology

Article Title: Brain-derived endothelial cells are neuroprotective in a chronic cerebral hypoperfusion mouse model

doi: 10.1038/s42003-024-06030-x

Figure Lengend Snippet: Vascular phenotypes in brains transplanted with ECs from different organs were analyzed on day 21 as described in Fig. . a Representative images show staining with anti-CD31 mAb (Alexa Fluor 647) and anti–claudin-5 pAb (Alexa Fluor 546) in regenerated vasculature transplanted with GFP-positive ECs derived from organs as indicated. The positive rate refers to the proportion of claudin-5–positive ECs among GFP-positive ECs expressed as a percentage. Scale bar, 30 μm. n = 5 per group. Data are presented as mean ± SEM. b , c Representative images stained with anti-CD31 mAb (Alexa Fluor 647), anti–NG2 pAb (Alexa Flour 546) ( b ), anti-CD31 mAb (Alexa Flour 647), and anti-GFAP pAb (Alexa Flour 546) ( c ) in regenerated vasculature areas transplanted with GFP-positive ECs derived from organs as indicated. The positive rate refers to the proportion of NG2-positive pericytes covering ECs ( b ) or astrocyte end-feet (GFAP-positive) positive ECs ( c ) among GFP-positive ECs expressed as a percentage. Scale bar, 30 μm. n = 5 per group. Data are presented as mean ± SEM. d Fluorescence images of vasculature transplanted with GFP-positive ECs derived from various organs as indicated. Blood flow was overlapped by labeling with AngioSPARK 680. The positive rate refers to the proportion of GFP-positive vessels with blood flow among GFP-positive ECs expressed as a percentage. Scale bar, 300 μm. n = 3 per group. Data are presented as mean ± SEM. *, P < 0.05; ***, P < 0.001; ****, P < 0.0001. EC endothelial cell, GFAP glial fibrillary acidic protein, GFP green fluorescent protein, mAb monoclonal antibody, NG2 neural/glial antigen-2, pAb polyclonal antibodies.

Article Snippet: For immunohistochemistry, the following antibodies were used: Armenian hamster anti-mouse CD31 mAb (MAB1398Z; Merck Millipore, Darmstadt, Germany; dilution 1/200), rabbit anti–claudin-5 pAb (polyclonal antibody; Abcam, Cambridge, MA, USA; dilution 1/200), rabbit anti-neural/glial antigen-2 (NG2) pAb (AB5320, Sigma–Aldrich, St. Louis, Missouri, USA; dilution 1/250), rabbit anti-glial fibrillary acidic protein (GFAP) pAb (HPA056030; Sigma–Aldrich; dilution 1/1000), Alexa Fluor 546–conjugated goat anti-rabbit IgG pAb (A-11010; Thermo Fisher Scientific; dilution 1/200), and Alexa Fluor 647–conjugated goat anti-Armenian hamster IgG pAb (127-605-160; Jackson ImmunoResearch Laboratories, West Grove, PA, USA; dilution 1/400).

Techniques: Staining, Derivative Assay, Fluorescence, Labeling

a Representative photographs of EC colonies. 5 × 10 3 ECs isolated from each organ as indicated were cultured on OP9 feeder cells for 10 days and stained with anti-CD31 antibody. Scale bar, 3 mm (low-power field) and 200 μm (high-power field). The mean vascular area generated by ECs from each organ ( n = 5 per group) was quantified. Data are presented as mean ± SEM. b Representative photographs of EC colonies. 5 × 10 3 ECs isolated from each organ as indicated were cultured with or without (control) fetal brain cells on OP9 feeder cells for 10 days and stained with anti-CD31 antibody. Scale bar, 3 mm (low-power field) and 200 μm (high-power field). The mean vascular area generated by ECs from each organ ( n = 5 per group) was evaluated. The vascular area with brain cells was compared to that without (control) brain cells. Data are presented as mean ± SEM. c Representative photographs of EC colonies. 5 × 10 3 ECs isolated from each organ as indicated were cultured with or without (control) the culture supernatant of fetal brain cells on OP9 feeder cells for 10 days and stained with anti-CD31 antibody ( d ). Scale bar, 3 mm. The mean vascular area generated by ECs from each organ ( n = 5 per group) was evaluated. The vascular area with the culture supernatant of brain cells were compared to that without supernatant. Data are presented as mean ± SEM. d Representative photographs of EC colonies. 5 × 10 3 ECs isolated from each organ as indicated were cultured with or without (control) astrocytes from fetal brain cells on OP9 feeder cells for 10 days and stained with anti-CD31 antibody. Scale bar, 3 mm. The mean vascular area generated by ECs from each organ ( n = 5 per group) was evaluated. The vascular area generated with astrocytes were compared to that without astrocytes. Data are presented as mean ± SEM. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns not significant. EC endothelial cell.

Journal: Communications Biology

Article Title: Brain-derived endothelial cells are neuroprotective in a chronic cerebral hypoperfusion mouse model

doi: 10.1038/s42003-024-06030-x

Figure Lengend Snippet: a Representative photographs of EC colonies. 5 × 10 3 ECs isolated from each organ as indicated were cultured on OP9 feeder cells for 10 days and stained with anti-CD31 antibody. Scale bar, 3 mm (low-power field) and 200 μm (high-power field). The mean vascular area generated by ECs from each organ ( n = 5 per group) was quantified. Data are presented as mean ± SEM. b Representative photographs of EC colonies. 5 × 10 3 ECs isolated from each organ as indicated were cultured with or without (control) fetal brain cells on OP9 feeder cells for 10 days and stained with anti-CD31 antibody. Scale bar, 3 mm (low-power field) and 200 μm (high-power field). The mean vascular area generated by ECs from each organ ( n = 5 per group) was evaluated. The vascular area with brain cells was compared to that without (control) brain cells. Data are presented as mean ± SEM. c Representative photographs of EC colonies. 5 × 10 3 ECs isolated from each organ as indicated were cultured with or without (control) the culture supernatant of fetal brain cells on OP9 feeder cells for 10 days and stained with anti-CD31 antibody ( d ). Scale bar, 3 mm. The mean vascular area generated by ECs from each organ ( n = 5 per group) was evaluated. The vascular area with the culture supernatant of brain cells were compared to that without supernatant. Data are presented as mean ± SEM. d Representative photographs of EC colonies. 5 × 10 3 ECs isolated from each organ as indicated were cultured with or without (control) astrocytes from fetal brain cells on OP9 feeder cells for 10 days and stained with anti-CD31 antibody. Scale bar, 3 mm. The mean vascular area generated by ECs from each organ ( n = 5 per group) was evaluated. The vascular area generated with astrocytes were compared to that without astrocytes. Data are presented as mean ± SEM. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns not significant. EC endothelial cell.

Techniques: Isolation, Cell Culture, Staining, Generated, Control

a 5 × 10 3 ECs from each organ as indicated were cultured on OP9 feeder cells with several doses of netrin-1 protein and stained with anti-CD31 antibody. Control represents no netrin-1 addition. Representative images are shown on the left. Scale bar, 3 mm. Quantitative evaluation is shown on the right. In each group, values were compared to the no addition of netrin-1 ( n = 3 per group). Data are presented as mean ± SEM. b 5 × 10 3 ECs from each organ as indicated were cultured on OP9 feeder and fetal brain cells, with and without anti–netrin-1 antibody, and stained with anti-CD31 antibody. Representative images are shown on the left. Scale bar, 3 mm. Quantitative evaluation is shown on the right. In each group, values were compared to no addition of brain cells or netrin-1 ( n = 3 per group). Data are presented as mean ± SEM. c Quantitative reverse transcription PCR analysis for netrin receptors (Unc 5A, Unc 5B, Unc 5C, Unc 5D, down syndrome cell adhesion molecule [DSCAM], deleted in colorectal cancer [DCC], and neogenin) in ECs from brain, liver, and fat ( n = 3 per group). Expression was relative to that of brain ECs. Data are presented as mean ± SEM. d 5 × 10 3 ECs from each organ as indicated were cultured on OP9 feeder and fetal brain cells, with and without soluble Unc 5B, and stained with anti-CD31 antibody. Representative images are shown on the left. Scale bar, 3 mm. Quantitative evaluation is shown on the right. In each group, values were compared to the no addition of brain cells or soluble UNC5B ( n = 3 per group). Data are presented as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns not significant. EC endothelial cell.

Journal: Communications Biology

Article Title: Brain-derived endothelial cells are neuroprotective in a chronic cerebral hypoperfusion mouse model

doi: 10.1038/s42003-024-06030-x

Figure Lengend Snippet: a 5 × 10 3 ECs from each organ as indicated were cultured on OP9 feeder cells with several doses of netrin-1 protein and stained with anti-CD31 antibody. Control represents no netrin-1 addition. Representative images are shown on the left. Scale bar, 3 mm. Quantitative evaluation is shown on the right. In each group, values were compared to the no addition of netrin-1 ( n = 3 per group). Data are presented as mean ± SEM. b 5 × 10 3 ECs from each organ as indicated were cultured on OP9 feeder and fetal brain cells, with and without anti–netrin-1 antibody, and stained with anti-CD31 antibody. Representative images are shown on the left. Scale bar, 3 mm. Quantitative evaluation is shown on the right. In each group, values were compared to no addition of brain cells or netrin-1 ( n = 3 per group). Data are presented as mean ± SEM. c Quantitative reverse transcription PCR analysis for netrin receptors (Unc 5A, Unc 5B, Unc 5C, Unc 5D, down syndrome cell adhesion molecule [DSCAM], deleted in colorectal cancer [DCC], and neogenin) in ECs from brain, liver, and fat ( n = 3 per group). Expression was relative to that of brain ECs. Data are presented as mean ± SEM. d 5 × 10 3 ECs from each organ as indicated were cultured on OP9 feeder and fetal brain cells, with and without soluble Unc 5B, and stained with anti-CD31 antibody. Representative images are shown on the left. Scale bar, 3 mm. Quantitative evaluation is shown on the right. In each group, values were compared to the no addition of brain cells or soluble UNC5B ( n = 3 per group). Data are presented as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns not significant. EC endothelial cell.

Techniques: Cell Culture, Staining, Control, Reverse Transcription, Expressing

a Quantification of vascular colony forming–areas by GFP-positive ECs. 5 × 10 3 CD157 + or CD157 - ECs from brains were seeded on OP9 feeder cells for 10 days and stained with anti-CD31 antibody ( n = 3 per group). b , c Vascular regeneration by transplanted CD157-positive or -negative ECs from GFP mice into a chronic cerebral hypoperfusion model. Representative images of vascular regeneration observed under TPEM in the same region using the same mice. b Vascular regeneration was evaluated on days 7 or 21 after transplantation. The bar graph shows the mean area (days 7, 21) of GFP-positive cells ( n = 4 per group) ( c ). Data are presented as mean ± SEM. d , e Rescue of brain fibrosis by CD157-positive ECs but not by CD157-negative ECs in a chronic cerebral hypoperfusion model. Representative images stained with anti-GFAP pAb (Alexa Flour 546). Scale bar, 50 μm. e Bar graphs showing the mean volume and total amount of fluorescence intensity of GFAP-positive cells ( n = 4 per group). Data are presented as mean ± SEM. f Bar graphs showing the mean novelty score on days 14 and 28 ( n = 4 per group) after the transplantation of ECs. Data are presented as mean ± SEM. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns not significant. EC endothelial cell, GFAP glial fibrillary acidic protein, GFP green fluorescent protein, pAb polyclonal antibody, TPEM two-photon excitation microscopy.

Journal: Communications Biology

Article Title: Brain-derived endothelial cells are neuroprotective in a chronic cerebral hypoperfusion mouse model

doi: 10.1038/s42003-024-06030-x

Figure Lengend Snippet: a Quantification of vascular colony forming–areas by GFP-positive ECs. 5 × 10 3 CD157 + or CD157 - ECs from brains were seeded on OP9 feeder cells for 10 days and stained with anti-CD31 antibody ( n = 3 per group). b , c Vascular regeneration by transplanted CD157-positive or -negative ECs from GFP mice into a chronic cerebral hypoperfusion model. Representative images of vascular regeneration observed under TPEM in the same region using the same mice. b Vascular regeneration was evaluated on days 7 or 21 after transplantation. The bar graph shows the mean area (days 7, 21) of GFP-positive cells ( n = 4 per group) ( c ). Data are presented as mean ± SEM. d , e Rescue of brain fibrosis by CD157-positive ECs but not by CD157-negative ECs in a chronic cerebral hypoperfusion model. Representative images stained with anti-GFAP pAb (Alexa Flour 546). Scale bar, 50 μm. e Bar graphs showing the mean volume and total amount of fluorescence intensity of GFAP-positive cells ( n = 4 per group). Data are presented as mean ± SEM. f Bar graphs showing the mean novelty score on days 14 and 28 ( n = 4 per group) after the transplantation of ECs. Data are presented as mean ± SEM. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns not significant. EC endothelial cell, GFAP glial fibrillary acidic protein, GFP green fluorescent protein, pAb polyclonal antibody, TPEM two-photon excitation microscopy.

Techniques: Staining, Transplantation Assay, Fluorescence, Microscopy

Development of a continuous imaging model of human blood vessels using human tumor tissues. ( a ) Human tumor was freshly resected from cancer patients. ( b ) Tumors were cut into 1–2 mm fragments with scissors. ( c ) Tumor fragments were transplanted into a cranial window in NOD-Scid mice. ( d ) Mice were intravenously injected with Alexa Fluor 488-conjugated anti-human CD31 monoclonal antibody (huCD31-AF488 Ab) to visualize the human blood vessels specifically in vivo. AF488-labeled human blood vessels were observed by confocal microscopy under isoflurane anesthesia.

Journal: Scientific Reports

Article Title: An in vivo model allowing continuous observation of human vascular formation in the same animal over time

doi: 10.1038/s41598-020-80497-6

Figure Lengend Snippet: Development of a continuous imaging model of human blood vessels using human tumor tissues. ( a ) Human tumor was freshly resected from cancer patients. ( b ) Tumors were cut into 1–2 mm fragments with scissors. ( c ) Tumor fragments were transplanted into a cranial window in NOD-Scid mice. ( d ) Mice were intravenously injected with Alexa Fluor 488-conjugated anti-human CD31 monoclonal antibody (huCD31-AF488 Ab) to visualize the human blood vessels specifically in vivo. AF488-labeled human blood vessels were observed by confocal microscopy under isoflurane anesthesia.

Article Snippet: Whole-mount tissue samples were stained with Armenian hamster anti-mouse CD31 mAb (MAB1398Z, Merck Millipore, Darmstadt, Germany, dilution 1/1000), and then stained with Alexa Fluor 647-conjugated anti-Armenian hamster pAb (127-605-160, Jackson ImmunoResearch Laboratories, West Grove, PA, dilution 1/1000).

Techniques: Imaging, Injection, In Vivo, Labeling, Confocal Microscopy

Determination of antibodies that specifically recognize human ECs. ( a ) Specificity of anti-CD31 antibodies for human ECs was analyzed by flow cytometry. Left panel shows unstained HUVECs (negative control) and right panel shows huCD31-AF488 Ab, moCD31-AF647 Ab double-stained HUVECs. ( b ) Specificity of anti-CD31 antibodies for mouse ECs (MS-1 cells) was analyzed by flow cytometry. Left panel shows unstained MS-1, and right panel shows huCD31-AF488 Ab, moCD31-AF647 Ab double-stained MS-1 cells. ( c ) Representative images of colorectal tumor tissues stained with huCD31-AF488 Ab (green). Nuclei (blue) were labeled with TO-PRO-3. Histograms and images are representative of three independent experiments.

Journal: Scientific Reports

Article Title: An in vivo model allowing continuous observation of human vascular formation in the same animal over time

doi: 10.1038/s41598-020-80497-6

Figure Lengend Snippet: Determination of antibodies that specifically recognize human ECs. ( a ) Specificity of anti-CD31 antibodies for human ECs was analyzed by flow cytometry. Left panel shows unstained HUVECs (negative control) and right panel shows huCD31-AF488 Ab, moCD31-AF647 Ab double-stained HUVECs. ( b ) Specificity of anti-CD31 antibodies for mouse ECs (MS-1 cells) was analyzed by flow cytometry. Left panel shows unstained MS-1, and right panel shows huCD31-AF488 Ab, moCD31-AF647 Ab double-stained MS-1 cells. ( c ) Representative images of colorectal tumor tissues stained with huCD31-AF488 Ab (green). Nuclei (blue) were labeled with TO-PRO-3. Histograms and images are representative of three independent experiments.

Techniques: Flow Cytometry, Negative Control, Staining, Labeling

VEGFR2 inhibition causes regression of human blood vessels. ( a ) Schema for tumor transplantation into the cranial window and ramucirumab or isotype IgG treatment. b-d, VEGFR2 inhibition decreases human blood vessels. Representative images of huCD31-AF488-positive human blood vessels (huCD31, green) ( b ). Quantification of total human blood vessel length ( c ) and relative length ( d ) after ramucirumab (Ram) or isotype IgG (Isotype) treatment. ( e – g ): Transplanted tissues were sectioned for the analysis of human and mouse blood vessels. Representative images for the expression of human CD31 (huCD31, green), mouse CD31 (moCD31, red), and/or human nuclei (HuNu, blue) ( e ). Quantification of the total length of human ( f ) and mouse ( g ) blood vessels. Data are mean ± SD. (isotype IgG: n = 5, ramucirumab: n = 4). Welch´s t test was used to compare with isotype IgG-treated mice. *: p < 0.05. **: p < 0.01. n.s.: not significant.

Journal: Scientific Reports

Article Title: An in vivo model allowing continuous observation of human vascular formation in the same animal over time

doi: 10.1038/s41598-020-80497-6

Figure Lengend Snippet: VEGFR2 inhibition causes regression of human blood vessels. ( a ) Schema for tumor transplantation into the cranial window and ramucirumab or isotype IgG treatment. b-d, VEGFR2 inhibition decreases human blood vessels. Representative images of huCD31-AF488-positive human blood vessels (huCD31, green) ( b ). Quantification of total human blood vessel length ( c ) and relative length ( d ) after ramucirumab (Ram) or isotype IgG (Isotype) treatment. ( e – g ): Transplanted tissues were sectioned for the analysis of human and mouse blood vessels. Representative images for the expression of human CD31 (huCD31, green), mouse CD31 (moCD31, red), and/or human nuclei (HuNu, blue) ( e ). Quantification of the total length of human ( f ) and mouse ( g ) blood vessels. Data are mean ± SD. (isotype IgG: n = 5, ramucirumab: n = 4). Welch´s t test was used to compare with isotype IgG-treated mice. *: p < 0.05. **: p < 0.01. n.s.: not significant.

Techniques: Inhibition, Transplantation Assay, Expressing

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